Beyond their impact on domesticated animals and humans, Brucella species also pose challenges in wildlife populations, with several species known to infect various mammals. Wild animals, such as elk, bison, and caribou, can serve as reservoirs for Brucella, contributing to the persistence of the bacteria in natural ecosystems. This creates a complex interface between wildlife, domestic animals, and human populations, presenting unique challenges for disease control. In conservation settings, the interaction between Brucella species and endangered wildlife raises concerns about the potential for disease transmission and population declines. The management of wildlife reservoirs, surveillance programs, and research into the ecology of Brucella in diverse ecosystems are crucial for understanding and mitigating the impact of these bacteria on biodiversity. Balancing the conservation of endangered species with the control of zoonotic diseases requires interdisciplinary collaboration between wildlife ecologists, veterinarians, and public health professionals. Strategies that promote healthy ecosystems, minimize human-wildlife conflict, and incorporate surveillance for Brucella species contribute to both wildlife preservation and the protection of human and animal health.