Title : Characteristic analysis for clinical isolates of Pseudomonas aeruginosa NCCP strains from Korea
Pseudomonas aeruginosa could be infected almost body tissues and is known to cause skin infections, endocarditis, pneumonia and bacteremia, sepsis diseases.
National Culture Collection for Pathogens (NCCP) in Korea is to distribute pathogen resources and provide their various information of quality assured to use to disease diagnostic researches and biomedical industry fields. There are 51 strains of P. aeruginosa registered and quality managed in NCCP with minimum species identification and culture condition information. Therefore, this study consisted of goals to distribute and provide various information to researchers through the characterization analysis of P. aeruginosa.
The analysis for antibiotic susceptibility, genotype, toxin type and serotype for of 51 P. aeruginosa NCCP strains were performed. Antibiotic susceptibility analysis (cefepime, gentamicin, imipenem, tobramycin, piperacillin-tazobactam, ceftazidime) was performed using the disk diffusion method according to the CLSI guidelines. Antibiotic susceptibility analysis revealed 18 groups, and 39.2% of P. aeruginosa showed non-susceptibility to all six antibiotics used in the experiment.
Sequence type analysis was performed by MLST to confirm the allele number of the housekeeping gene and the result was confirmed by PubMLST (https://PubMLST.org). 26 groups were classified and ST 235 was the most common at 37.3%. Toxin type analysis was performed by PCR of toxin gene exo (S, T, U, Y) and toxA. All strains contained more than one toxin gene, and P. aeruginosa showed 98%, 51%, 92.2%, 51% and 88.2% of toxA, exoS, exoT, exoU and exoY genes, respectively. For serotype analysis, A ~ N type was analyzed using Pseudomonas aeruginosa antisera commercial kit. The serotype analysis revealed that 8 of the 14 serotypes were present. The most common serotype was E type (45.1%) and untype (23.5%) followed by B (7.8%), A (5.9%), G (5.9%), L (5.9%), I (2%), K (2%) and M (2%).
We expect that our characteristic data and assured resources are provided and used as the valuable standard materials to develop disease diagnosis or therapeutic research fields.
This study was supported by the funds of the Korea Centers for Disease Control & Prevention, Ministry of Health and Welfare (Funding no. 2019-NG046-00).