Title : Etiologic involvement of enterovirus and human bocavirus in acute flaccid paralysis cases in India
Abstract:
Background: Acute flaccid paralysis (AFP), characterized by the rapid onset of asymmetric paralysis, can be caused by a variety of viral infections or coinfections. The clinical manifestations range from conjunctivitis, respiratory tract infection, myocarditis, meningitis, encephalitis, and neonatal sepsis-like illness. Human Bocavirus (HBoV), a newly classified member of the Parvoviridae family, has possible etiological involvement.
Methods: Total 586 stool specimens were collected and VP1 capsid region, used for detection of human enteroviruses (HEV), human boca viruses (HBoV) and saffold viruses in direct clinical specimen.
Results: HEV RNA was detected in 103 (17.6%) by targeting 5' UTR region. Out of them, 71 (12.11%) were NPEV, partially sequenced by VP1 which revealed the prevalence of echovirus (ECV) 19 (n = 6), ECV 11 (n = 7), ECV 18 (n = 4), ECV 33 (n = 5), ECV 29 (n = 1), ECV 25 (n = 2), ECV 24 (n = 3), ECV 3 (n = 3), ECV 14 (n = 2), ECV 13 (n = 1), ECV 2 (n = 1), ECV 20 (n = 2), ECV 27 (n = 4), ECV 6 (n = 2), CV A10 (n = 2), CV A9 (n = 1), CV A6 (n = 2), CV B4 (n = 1), CV B5 (n = 3), CV B6 (n = 3), EV 80 (n = 1), EV 83 (n = 1), EV 97 (n = 2).
Total 63 (10.75%) HBoVs were detected, consists of HBoV-1 (n = 8), HBoV-2 (n = 15), HBoV-3 (n = 9) and HBoV-4 (n = 5). Out of them 9 (1.5%) were in co-infection with NPEVs and showed 0.9 - 5.6% divergence at nucleotide level. Total 9 (1.5%) Saffold viruses was detected and characterized by VP1 sequencing.
Conclusion: Molecular typing of these viruses is useful for characterizing emerging serotypes and their epidemiological investigation
