Title : Whole genome sequencing for genetic analysis of virulence factors of nosocomial extensively resistant Klebsiella pneumoniae isolated from different clinical specimens from ICUs
Abstract:
Klebsiella pneumoniae is the most common pathogen causing nosocomial infections owing to their potent virulence factors Viz. capsule, Fimbriae, siderophores, Efflux pump, regulatory secretions & secretory system. Since most of the nosocomial K. pneumoniae infections are MDR/XDR the treatment choice has narrowed down over time and very few options are left. It is imperative to search for other modalities of tackling this potent pathogen basically hindering the virulence factors pathways. With that view the present study comprised of study of various virulence factors by whole genome sequencing. A total of 188 K. pneumoniae isolates from various nosocomial infections were collected, identified by VITEK 2 as well as MALDI-TOF-MS. The present study includes 42 isolates that were subjected to WGS for study of different virulence factors.
The genes for Type I fimbriae were found in all clinical specimens. Variation is seen in type III fimbriae found in isolated from Blood stream Infections. Besides the virulence factors seen in other Enterobacterals, the K. pneumoniae also possessed virulence factors like enterobactin siderophore, aerobactin, salmochelin & yersiniabactin siderophores. Additional secretory system Type 1 and 3 was detected in our strains and variation was observed in Type 2.
