Title : To study the bacteriological episodes of urinary tract infection in different age group with relation to gender

Abstract:

A urinary tract infection is a condition in which one or more parts of the urinary system (kidneys, ureters, bladder, and urethra) become infected. Urinary tract infection may be defined as the presence of bacteria undergoing multiplication in urine within the urinary drainage system. A count 105 organism/ml of urine denotes significant bacteriuria and indicate active urinary tract infection. Moreover, urinary tract infection is generally caused by one species, while contaminants are generally of mixed species. Urinary tract infection is one of the commonest bacterial infections. The Enterobacteriaceae are the most frequent pathogen detected causing 80% of urinary tract infection. Present study was conduct to achieve resistance summary of clinical isolates against commonly prescribed antibiotic.

A total of 100 urine specimens were received and these were processed in the laboratory. Significant bacteria: (cultures with > 10 ⁵ colony forming units (CFU) of bacteria/ml of urine). Gram-negative bacteria were more prevalent than Gram-positive bacteria. Identification was done on the basis of morphological, biochemical and phenotypic characteristics of the 100 isolates, the most commonly isolated bacteria were Escherichia coli 45, Klebsiella pneumoniae 22, Pseudomonas aeruginosa 19. The study shows the distribution of microbial species isolated from patients with urinary tract infection and their susceptibility pattern to antimicrobial agents. Microbial infection of the urinary tract infection is one of the most common infectious diseases worldwide. The present study was to describe the isolation and identification of Pseudomonas sp.,

Klebsiella sp. and E. coli in urinary tract infection patients and to study antibiotic susceptibility patterning of these as well. As E.coli is first major pathogen that may lead to UTI after its Klebsiella and Pseudomonas play role. Out of 100 samples, 19 samples were isolated as Pseudomonas sp. and 22 Klebsiella and E. coli 47. The percentage of resistance by antibiotics were Amikacin (21.05%), Ciprofloxacin (21.05%), Gentamicin (10.52%), Cefotaxime (84.21%), Imipenem (5.2%), Meropenem (10.52%), Cefoperazone (0%), Tobramycin (5.26%), Piperacillin-tazobactam (5.26%), Cefepime (10.52%), Ceftazidime (31.57%), Norfloxacin (63.15%).

Audience Take Away:

• To isolate and identify the pathogenic bacteria in urine sample.
• To perform antibiotic susceptibility test of bacterial isolates.
• To estimate the prevalence of Multi Drug Resistant microorganism from the isolates.

Biography:

Ms. Shikha studied M.Sc. in Clinical Microbiology at the Lovely Professional University, Phagwara, Punjab, India. She received her Post Graduated degree in 2018 at the same University. After that she obtained the position of an Assistant Professor. From last 5.5 years, she is working as an Assistant Professor and presently she is working at GNA University. She is Board of Studies (BOS) member, Event coordinator and clinical laboratory in-charge. She has published 4 research papers in various reputed journals. She has attended prestigious conferences, faculty development programs, expert talks and seminars. Other than this, she attended and organized medical camps and workshops. Moreover, she participated in oral paper presentation at international conferences and bestowed with awards.