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WCID 2024

Coffee industry by-products extracts: Evaluation of the repellent effect against the R (B.) microplus tick

Rosemeire Cristina Linhari Rodrigues Pietro, Speaker at Infection Conferences
School of Pharmaceutical Sciences, Brazil
Title : Coffee industry by-products extracts: Evaluation of the repellent effect against the R (B.) microplus tick

Abstract:

Livestock plays a crucial role in the Brazilian economy, however, the ubiquitous presence of the Rhipicephalus (Boophilus) microplus tick represents a significant challenge, resulting in considerable economic losses. Additionally, ticks stand out as vectors of different microorganisms for humans and animals, such as Lyme disease, Babesiosis, Anaplasmosis, Tularemia, and Rocky Mountain spotted fever. This parasite not only impacts animal production, but also increases veterinary care costs, and excessive use of commercial acaricides leads to the development of resistance.

The coffee industry generates by-products that offer an opportunity to explore new repellents. These by-products offer a unique opportunity to explore new tick repellents. In the phytotherapeutic scenario, herbal medicine emerges as a promising alternative for tick repellence. The objective of this study was to evaluate hydroethanolic extracts from by-products of the coffee industry, such as defective roasted beans, defective green beans and leaves, for their repellent effects against R. (B.) microplus. To obtain the extracts, the sonication process was carried out with EtOHH2O 7:3 (v/v), using 500mg of sample for 5mL (plant material/solvent). The repellent activity was evaluated according to the methodology described in the literature.

The extracts were solubilized in Milli-Q water and Tween 2%, at a concentration of 25 mg/mL, and were tested at three-time intervals: 4, 12, and 24 hours of the extracts against ticks. The green grain and leaf extract only showed effects within 4 hours, with 0.6% and 0.7% repellence, respectively. The green grain extract demonstrated effects at intervals of 4 and 12 hours, with 7.8% and 0.6% repellence, respectively. The repellent effects of coffee by-product extracts provide insights for future research at different concentrations, methods of obtaining plant extracts, and tested time intervals, as well as potential associations with biotechnological products. This approach aims to enhance the repellent percentage, enabling its use in tick control in cattle farming.

Audience Take Away: 

  • The audience will learn about the potential repellent effects of hydroethanolic extracts from coffee industry by-products against the Rhipicephalus (Boophilus) microplus tick.
  • They will understand the methodology used in the study, including the extraction process and the testing procedure for repellency.
  • The presentation will highlight the specific results obtained from testing different extracts at various time intervals, providing information on the efficacy of these extracts as tick repellents.
  • Attendees will gain knowledge about the potential applications of coffee by-product extracts in tick control and the need for further research to optimize their effectiveness.
  • Veterinarians and livestock farmers can explore alternative methods for tick control, potentially reducing reliance on conventional acaricides and mitigating resistance development.
  • Researchers in the field of veterinary medicine and agriculture can use this research as a basis for further studies on natural repellents and their application in pest management.
  • The findings may inspire collaboration between the coffee industry and livestock sectors to develop ecofriendly solutions for tick control, benefiting both industries and the environment.
  • This research contributes to the development of sustainable and cost-effective strategies for tick management, ultimately improving animal health and reducing economic losses in the livestock industry. This research provides:
  • A potential practical solution to the challenge of tick infestation in livestock, offering a natural alternative to conventional acaricides.
  • Opportunities for interdisciplinary collaboration between the agriculture and food industries, fostering innovation and sustainability. - Insights into the utilization of agricultural by-products for pest management, promoting waste reduction and circular economy principles.
  • New information on the repellent properties of coffee industry by-products, expanding the repertoire of natural pest control options for farmers and veterinarians.
  • A foundation for future research endeavors aimed at optimizing the efficacy and applicability of coffee by-product extracts in tick control strategies.

Biography:

She holds a degree in Biochemical Pharmacy from São Paulo State University Júlio de Mesquita FilhoUNESP (1978), a master's degree in Biochemistry from the University of São Paulo-USP (1985), a Ph.D. in Biochemistry from the University of São Paulo-USP (1991), and a post-doctorate from the University of São Paulo-USP (1995). He is currently an associate professor at São Paulo State University Júlio de Mesquita Filho-UNESP, at the Faculty of Pharmaceutical Sciences (FCFAR), in the Department of Drugs and Medicines, at the Pharmaceutical Biotechnology Laboratory. She was the coordinator of the Graduate Program in Pharmaceutical Sciences at FCFAR from 2013 to 2021. She is a Full Member of the Central Postgraduate Chamber-CCPG (2017-2019), UNESP. She was President of the Permanent Teaching Commission of FCFAr-UNESP.

Full Member of the Congregation (2009-2024). She is the leader of the CNPq research group: Biotechnological Aspects of Studies of Enzymatic, Antimicrobial, and Biological Activities. Full Member of the Departmental Council of the Department of Drugs and Medicines FCFAR (2008 - 2016; 2018 - currently), being a substitute member from 2016 to 2018. Full Member of the Council of the Graduate Program in Pharmaceutical Sciences, FCFAR (2013-currently). She has experience in the area of Biochemistry, with emphasis on Microorganism Biochemistry and Biotechnology, mainly working on the following themes: enzymes, antimicrobial activity, acaricidal activity, plant extracts, natural products, and microbiological analyses of biotechnological products.

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