Title : Enhancing anti-Folic acid efficacy of Cotrimoxazole with a Nano-stabilizing agent {Al4 (SiO4)3 + 3Mg2SiO4 ? 2Al2 Mg3 (SiO4)3} for a new anti-trypanosmosis drug.

Abstract:

Negatively charged ends and positively charged ends that coexist on Nano-particles of Aluminum-magnesium silicate (AMS), a WHO approved medicine, make them to hydrate and form three dimensional colloidal structures, when in solution. The colloidal structures stabilize other drugs, formulated with AMS. Also, Nanoparticles enhance drugs` delivery to effect-targets and across physiological barriers. When drugs are stabilized, rate of their degradation in blood reduces, thus prolonging time they remain at high bioavailability. Prolonging time of high bioavailability and enhancing delivery to targets improve efficacy. With improved efficacy, lower doses achieve desired effects. Use of lower doses for desired effects, minimizes side effects so that immune responses improve. Synergy between improved efficacies and enhanced immune responses, lead to clearance of infections to prevent Antimicrobial Resistance. Even already resistant infections can be cured by same drugs being resisted. Some countries do not have natural deposits of AMS [Al₂Mg₃ (SiO₄)₃] but they may have Aluminum silicate [AS: Al₄ (SiO₄)₃] and Magnesium silicate [MS: Mg₂SiO₄]. So, we used AS and MS (approved medicines, too) to formulate AMS-brand {Al₄ (SiO₄)₃ + 3Mg₂SiO₄ → 2Al₂ Mg₃ (SiO₄)₃} and named it, Medicinal synthetic AMS {MSAMS}. Since protozoa need Folic acid for replication, anti-Folic acid efficacy of Cotrimoxazole was exploited to develop a new drug for trypanosmosis (a debilitating tropical disease of man and animals). Cotrimoxazole was stabilized with MSAMS so that its anti-Folic acid efficacy may improve. The drug alone, significantly (P≤0.05) reduced trypanosome-parasitemia in mice from 12.76±1.20 to 5.86± 0.43 while the Cotrimoxazole-MSAMS formulation achieved 0.00±00 parasitemia.  In infected sheep treated with the Cotrimoxazole-MSAMS formulation, mean trypanosome parasitemia (1.00±0.00) was significantly (P≤0.05) lower than 81.60±27.71 of an untreated group, two days post treatment, while by nine days post treatment, the parasitemia had completely cleared and there was no relapse in sheep monitored for 70 days, post treatment.

Biography:

Maduike Ezeibe holds PhD, from University of Nigeria, Nsukka. He specialized in using animals for medical researches and invented theory of opposite charges electrostatic attraction for treatment of diseases of electrically charged pathogens. Since Aluminum-magnesium silicate (AMS) which has both charges may not exist in every country, he invented a formulation of Aluminum silicate and Magnesium silicate (approved medicines) to get Medicinal Synthetic AMS (MSAMS) and also invented an equation {Al₄ (SiO₄)₃ + 3Mg₂SiO₄ →2Al₂ Mg₃ (SiO₄)₃} for the formulation. MSAMS has proved effective against viral/abnormal cell diseases. It also enhances efficacy of other medicines to prevent/cure Antimicrobial Resistant infections.