Title : Subgingival and ruminal microbiomes associated with bovine periodontal disease
Periodontal diseases (gingivitis, chronic or aggressive periodontitis, necrotizing periodontal disease and periodontal abscess) are a group of diseases that affect the protection and support structures of the dental elements and are associated with etiological, environmental and systemic factors. While bacterial involvement in the pathogenesis of periodontal disease is well recognized, the pathogens that contribute to the development of the disease are not clearly defined. On the other hand, it is believed that so far only 10% of rumen bacterial species have been identified and little is known about interaction of rumen and oral microbiota and whether or not the microorganisms present in rumen microbiota would contribute to the development of the periodontal disease in ruminants. Thus, the present study aimed to identify the subgingival and ruminal microbiomes associated with periodontitis in cattle bred extensively in the Amazon region of Brazil. Clinical status of 334 cattle in different farms of Amazon region was established by oral examination and periodontal evaluation and subgingival biofilm and ruminal content samples were obtained from animals with periodontal disease (21 biofilm and 13 ruminal content samples) and of animals considered periodontally healthy (25 biofilm and 9 ruminal content samples) with the aid of a sterile curette. Amplicon libraries were sequenced at the Environmental Sample Preparation and Sequecing Facility (ESPSF) at Argonne National Laboratory using a barcode primer set adapted for the Illumina HiSeq2000 and MiSEq platform that targets the V4 region of the 16S rRNA gene (517F-806R).