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Potential speaker for Infectious Diseases conferences 2020
Ilya Kandinov
Russian Academy of Sciences, Russian Federation
Title : Molecular drug susceptibility testing and genotyping of Neisseria gonorrhoeae isolates in Russia

Abstract:

Gonococcal infection belongs to the most common sexually transmitted infections. Due to the developing antimicrobial resistance, in particular, resistance to fluoroquinolones and 3rd generation cephalosporins, Neisseria gonorrhoeae is considered to be a high priority pathogen according to the WHO list.

In this work, we analyzed 270 N. gonorrhoeae clinical isolates collected in Russia in 2018–2019. The phenotypic susceptibility testing was performed using agar dilution method. Hydrogel microarray with immobilized oligonucleotide probes was developed for the identification of genetic markers causing the N. gonorrhoeae resistance to penicillins, fluoroquinolones, and cephalosporins: mutations in the penA gene encoding penicillin-binding protein PBP2 (Ala311Val, Ile312Met, Val316Thr/Pro, ins345Asp, Thr483Ser, Ala501Val/Pro/Thr, Asn512Tyr, Gly545Ser, Pro551Ser/Leu), ponA gene (Leu421Pro), mtrR promoter region (-35delA, -10insT), porB gene (Gly120Lys/Asp/Asn/Thr, Ala121Ala/Asp/Asn/Gly), gyrA gene (Ser91Phe/Thr, Asp95Ala/Gly), and parC gene (Ser87Arg/Asn/Ile, Glu 91Lys). The mutations in the penA gene, which are analyzed using microarray, can identify the presence of the mosaic penA alleles and alterations associated with resistance to 3rd generation cephalosporins. For validation microarray results and genotyping (N. gonorrhoeae multiantigen sequence typing, NG-MAST) Sanger sequencing was performed.

Biography:

Ilya Kandinov, age 23, is a post-graduate student at the Engelhardt Institute of Molecular Biology, Russian Academy of Sciences (EIMB, study supervisor – Dr. Boris Shaskolskiy). Ilya has been working in the EIMB since 2018. He has obtained a Master’s degree in bioengineering and bioinformatics in 2019. He has graduated from the I.M. Sechenov First Moscow State Medical University of the Ministry of Health of the Russian Federation (Sechenov University). The research interests of Ilya Kandinov include specialized DNA microarrays design, state-of-the-art technologies in genomic engineering and molecular diagnostics, study of molecular mechanisms of drug resistance in bacteria and viruses, molecular evolution and phylogeny of Neisseria gonorrhoeae. Ilya Kandinov has authored 4 publications in peer-reviewed scientific journals.

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