Title : Infections caused by antibiotic-resistant ESKAPE group in Mexico
Infections caused by antibiotic-resistant ESKAPE group microorganisms possess high risk to patients' lives, because treatment is limited to few therapeutic options. The resistance mechanisms are: production of Extended Spectrum Lactamases (BLEE), Carbapenemes type MBL, enzymatic production of aminoglycoside resistance (HLRA), resistance to vancomycin and methicillin by modification of target site. In Mexico, epidemiological surveillance as well as control and prevention measures are regulated for the control and prevention of nosocomial infections. The American Society of Infectious Diseases (IDSA) defined a group of bacteria included in the term ESKAPE: Enterococcus faecium resistant to vancomycin (ERV), methicillin-resistant Staphylococcus aureus (MRSA), Klebsiella pneumoniae with expected -extended spectrum lactamic (BLEEs), Acinetobacter baumannii, Pseudomonas aeruginosa and Enterobacter species, as high-priority pathogens, for representing relevant clinical or public health problems, as well as being very hard therapeutic alternatives.
In this talk I would like to present experiences and work of ours staff group and some other groups have done in Mexico, where the ESKAPE group is already being studied. The audience will be able to learn what methods we suggest to detect BLEEs, AmpC production by inhibition with fenil-boronic-acid and how to be confirmed by PCR assay. Metallo-β-lactamases-(MBL) phenotype production determined by disk diffusion with EDTA. Genes blaKPC, blaVIM, blaIMP, blaNDM and blaOXA-24-like with PCR and efflux pumps using inhibitor of efflux pumps CCCP. Clonality analysis by PGE dissemination in all floors of hospitalization. Our knowledge help to understand the accuracy of results and provide information to assist and resolve similar problems and stablish prevention methods as hand washing.